Publications & News

 

News:

  • Miniature Synchrotron Sheds New Light on Research, RSNA View Article. June 2006
  • "Accelerated Technologies Center for Gene to 3D Structure" funded by the National Institute of Health, Institute of General Medical Sciences' Protein Structure Initiative and the National Center for Research Resources View more June 2005

  • Automating, Miniturizing X-ray Crystallography, Drug Discovery & Development, View article. March 2005

  • First complete data set collected using in-situ x-ray diffraction on capillary grown crystals at GM/CA CAT. In collaboration with the NIGMS/NCI funded GM/CA CAT at APS, first complete, high-resolution data sets were collected in March 2005. The crystals were grown in 0.2 mm diameter capillaries at the Ismagilov lab with proteins provided by the Kuhn-Stevens labs at Scripps. March 2005

  • Compact Light Source in component commissioning phase; First protein diffraction experiments scheduled for May/June 2005

  • First in-situ x-ray diffraction experiments from capillary based microfluidics experiments at APS GM/CA CAT beamline completed in Dec 2004.

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Publications

2005:

D.C. Carter, P. Rhodes, D.E. McRee, L. Tari, D.R. Dougan, G. Snell, E. Abola, R.C. Stevens "Reduction in diffuse-convective disturbances in nanovolume protein crystallization experiments" J. Appl. Cryst. 38, 87-90 (2005).[PDF]

R. Page, S. Lesley, A. Deacon, R. C. Stevens "Shotgun Crystallization Strategy for Structural Genomics II: Crystals and Conditions that Produce High Resolution Structures for T. maritima Proteins" J. Funct. Struct. Genomics, in press (2005).

R. Page, W. Peti, K. Moy, I. Wilson, R. Stevens, K. Wuthrich "NMR Screening of Mouse Homolog Proteins in a Structural Proteomics Pipeline" Proc. Natl. Acad. Sci. USA, 102, 1901-1905 (2005).[PDF]

B. L. Staker, M. D. Feese, M. Cushman, Y. Pommier, D. Zembower, L. Stewart, and A. B. Burgin (2005). Structures of Three Classes of Anticancer Agents Bound to the Human Topoisomerase I-DNA Covalent Complex J. Med. Chem. in press.

L. Stewart and Alex B. Burgin (2005). Whole Gene Synthesis: A Gene-O-Matic Future. In Frontiers in Drug Design and Discovery. Bentham Science Publishers, Ltd, Co-Editors Atta-ur-Rahman, B. A. Springer, G. W. Caldwell. in press.

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2004:

M. Bern, D. Goldberg, R.C. Stevens, P. Kuhn "Automatic classification of protein crystallization images using a curve tracking algorithm" J. Appl. Cryst., 37, 279-287 (2004). [PDF]

J.M. Canaves, R. Page, R.C. Stevens "Protein biophysical properties that correlate with crystallization success in Thermotoga Maritima: Maximum clustering strategy for structural genomics" J. Molec. Biol., 344, 977-991 (2004). [PDF]

Chrencik, J. E., B. L. Staker, A. B. Burgin, P. Pourquier, Y. Pommier, L. Stewart, M. R. Redinbo (2004). "Mechanisms of camptothecin resistance by human topoisomerase I mutations". J. Mol. Biol.339:773-784.

D.E. Kim, D. Chivian, D. Baker. “Protein structure prediction and analysis using the Robetta server.” Nucleic Acids Res., 32(Web Server issue), W526-W531 (2004). [PDF]

Nollert, P. and L. Stewart. "Climbing Mount Everest, the GPCR way". Drug Discovery Today3:1-4. (2004)

R. Page, R.C. Stevens "Crystallization data mining in structural genomics: using positive and negative results to optimize protein crystallization screens". Methods, 34, 373-389 (2004). [PDF]

R. Page, K. Moy, E.C. Sims, J. Velasquez, B. McManus, C. Grittini, T.L. Clayton, R.C. Stevens "Scalable high-throughput micro-expression device for recombinant proteins" BioTechniques, 37, 364-370 (2004) [PDF]

D. Pantazatos, J.S. Kim, H.E. Klock, R.C. Stevens, I.A. Wilson, S.A. Lesley, V.L. Woods Jr "Raid refinement of crystallographic protein construct definition employing enhanced hydrogen/deuterium exchange MS" Proc. Natl. Acad. Aci U.S.A., 101, 757-762 (2004). [PDF]

B. Qian, A. Ortiz, D. Baker. “Improvement of Comparative Model Accuracy by Free Energy Optimization along Principal Components of Natural Structural Variation.” Proc. Natl. Acad. Sci. U.S.A., 101, 15346-15351 (2004). [PDF]

G. Snell, C. Cork, R. Nordmeyer, E. Cornell, G. Meigs, D. Yegian, J. Jaklevic, J. Jin, R.C. Stevens, T. Earnest "Automated sample mounting and alignment system for biological crystallography at a synchrotron source" Structure, 12, 537-545 (2004). [PDF]

R.C. Stevens. "Long Live Structural Biology" Nature Struct. Molec. Biol., 11, 293-295 (2004). [PDF]

B. Taneri, B. Snyder, A. Novoradovsky, T. Gaasterland. “Alternative splicing of mouse transcription factors affects their DNA-binding domain architecture and is tissue specific.” Genome Biol. 5, R75 (2004). Epub 2004 Sep 30.[PDF]

B. Zheng, J.D. Tice, L.S. Roach, R.F. Ismagilov. “A droplet-based, composite PDMS/glass capillary microfluidic system for evaluating protein crystallization conditions by microbatch and vapor-diffusion methods with on-chip x-ray diffraction.” Angew. Chem. Int. Edit., 43, 2508-2511 (2004). [PDF]

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2003:

D. Hosfield, J. Palan, M. Hilgers, D. Scheibe, D. McRee, R.C. Stevens. "A Fully Integrated Protein Crystallization Platform for Small Molecule Drug Discovery" J. Struct. Biol. 142, 207-217, (2003). [PDF]

R. Page, S.K. Grzechnik, J. Canaves, G. Spraggon, A. Kreusch, R.C. Stevens, S. Lesley. "Shotgun crystallization strategy for structural genomics: An optimized two-tiered crystallization screen against the Thermatoga maritima proteome". Acta Crystallogr. D, D59, 1028-1037 (2003).[PDF]

R.C. Stevens. "The cost and value of three-dimensional protein structure", Drug Discovery World, 4, 35-48 (2003). [PDF]

M. Weselak, M.G. Patch, T.L. Selby, G. Knebel, R.C. Stevens "Robotics for Automated Crystal Formation and Analysis" In: Methods in Enzymology. Eds. C.W. Carter & R.M. Sweet. Academic Press: San Diego CA. Volume 368: 45-76 (2003).

B. Zheng, L.S. Roach, R.F. Ismagilov. “Screening of protein crystallization conditions on a microfluidic chip using nanoliter-size droplets.” J. Am. Chem. Soc., 125, 11170-11171 (2003). [PDF]

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2002:

P. Kuhn, K. Wilson, M.G. Patch, R.C. Stevens "The genesis of high-throughput structure-based drug discovery using protein crystallography" Curr. Opin. Chem. Biol., 6, 704-710 (2002). [PDF]

S.A. Lesley, P. Kuhn, A. Godzik, A.M. Deacon, I. Mathews, A. Kreusch, G. Spraggon, H.E. Klock, D. McMullan, T. Shin, J. Vincent, A. Robb, L.S. Brinen, M.D. Miller, T.M. McPhillips, M.A. Miller, D. Scheibe, J.M. Canaves, C. Guda, L. Jaroszewski, T.L. Selby, M.A. Elsliger, J. Wooley, S.S. Taylor, K.O. Hodgson, I.A. Wilson, P.G. Schultz, R.C. Stevens "Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline" Proc. Natl. Acad. Sci. USA, 99, 11664-11669 (2002). [PDF]

T.M. McPhillips, S.E. McPhillips, H.J. Chiu, A.E. Cohen, A.M. Deacon, P.J. Ellis, E. Garman, A. Gonzalez, N.K. Sauter, R.P. Phizackerley, S.M. Soltis, P. Kuhn. “Blu-Ice and the distributed control system: software for data acquisition and instrument control at macromolecular crystallography beamlines.” J. Synchrotron Radiat., 9, 401-406 (2002). [PDF]

B.D. Santarsiero, D.T. Yegian, C.C. Lee, G. Spraggon, J. Gu, D. Scheibe, D.C. Uber, E.W. Cornell, R.A. Nordmeyer, W.F. Kolbe, J. Jin, A.L. Jones, J.M. Jaklevic, P. G. Schultz, R.C. Stevens "An approach to rapid protein crystallization using nanodroplets" J. Appl. Crystallography, 35, 278-281 (2002). [PDF]

T.L. Selby, R.C. Stevens "Bioinformatics and high-throughput protein production for structural genomics" In: Gene Cloning and Expression Technologies, M.P. Weiner & Q. Lu (Eds)., Westborough, MA: Biotechniques Press, 281-304 (2002). [PDF]

Staker, B. L., K. Hjerrild, M. D. Feese, A. B. Burgin Jr., and L.Stewart. "The mechanism of topoisomerase I poisoning by a camptothecin analog." Proc. Natl. Acad. Sci. USA 99:15387-15392. (2002)

L. Stewart, R. Clark, C. Behnke. “High throughput crystallization and structure determination in drug discovery.” Drug Discovery Today 7, 187-196 (2002). [PDF]

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2001:

K.E. Goodwill, M.G. Tennant, R.C. Stevens. "High-throughput X-ray Crystallography for Structure-based Drug Design". Drug Discovery Today, 6, S113-8 (2001). [PDF]

R.C. Stevens, S. Yokoyama, I.A. Wilson. "Global efforts in structural genomics." Science, 294, 89-92 (2001). [PDF]

R.C. Stevens, I.A. Wilson "Industrializing structural biology" Science, 293, 519-520 (2001). [PDF]

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2000:

E. Abola, P. Kuhn, T. Earnest, R.C. Stevens "Automation of X-ray Crystallography" Nature Struct. Biol., 7, 973-977 (2000).[PDF]

H. Erlandsen, E. Abola, R.C. Stevens " Combining Structural Genomics and Enzymology: Completing the Picture in Metabolic Pathways and Enzyme Active Sites" Curr. Opin. Struct. Biol., 10, 719-730 (2000). [PDF]

R.C. Stevens, " High-throughput protein crystallization" Curr. Opin. Struct. Biol., 10, 558-563 (2000). [PDF]

R.C. Stevens "Design of High-throughput Methods of Protein Production for Structural Biology" Structure Folding and Design, 8, R177-R185, (2000). [PDF]

1998:

Z. Huang, R.D. Ruth. “Laser-electronic storage ring.” Phys. Rev., 80, 976-979 (1998).

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Patents:

“Robot for Mixing Crystallization Trial Matrices” U.S. Patent No. 6,818,062. Issued November 16, 2004. Inventors: Lance Stewart, Alexandrina Muntianu, and Robin Clark.

“Crystallization Media” U.S. Patent No. 6,267,935. Issued July 31, 2001. Inventors: Wim G. J. Hol, Steven H. Sarfaty, Lance Stewart, and Hidong Kim.

“Crystallization Tray” U.S. Patent No. 6,039,804. Issued March 21, 2000. Inventors: Hidong Kim and Lance Stewart.